FITC抗體標誌套組
只需一個步驟
不須Dialysis或desalt步驟。效能近乎100%。
(貨號#707-0010)
Fluorescein ( FITC )抗體標誌directly labeling kit (貨號#707-0010)，一定選擇英國innova biosciences公司專利研發之lightning-link套組。市面上antibody labeling kit，在pH8~9環境下做反應。如此地環境易造成抗體活性失效，即使照著原廠提供的Protocol下進行反應，抗體回收的效能也很差。因為多太抗體未被標誌，必須移去未結合的抗體。如此浪費珍貴的抗體。
英國INNOVA BIOSCIENCES，以生物結合為主要事業。於1996年專利研發，中性環境下進行抗體結合反應，只要一個步驟，30秒hands on time。其抗體回收效能近似100%。不須再做Dialysis或desalt的步驟。
Lightning-Link 只要一個步驟，即可將蛋白質/抗體/胜肽做標誌，具專利研發Quencher reagent化學反應徹底中止殘餘反應及停止非專一性結合，因此無須desalt及dialysis，抗體標誌回收率100%。
Lightning-link抗體標誌原理:
Lightning-Link以共價鍵結，辨視amino groups，性質極為穩定，一次反應後，可冷凍保存，節省實驗時間。
FITC螢光dye特性
Fluorescein is a fluorophore widely used in biological analysis, with a pH dependent absorption and emission spectrum. In water it shows an absorption maximum at 494 nm and an emission maximum of 520 nm.
Lightning-link螢光蛋白抗體標誌---期刊發表:
Lightning-Link Cy5---期刊發表
Lightning-link抗體標誌V.S.保存液:
抗體保存於Azide、Gelatin及BSA溶液下，會干擾一般結合的效果，所以會再經過純化步驟，浪費許多抗體。Lightning-Link克服Azide、Gelatin及BSA干擾結合的效率，省下您寶貴的抗體。別於其它結合的方式，Lightning-Link無須在High pH的溶液下操作，所以只要是具有amino groups (lysin/a-amino)的蛋白質/胜肽/抗體，都可反應。
Lightning-link抗體標誌應用:
可以應用在ELISA. immunofluorescent. Flow. confocol. Western Blot等檢診及分子病毒之研究!!直接在一抗上做標誌 (可標誌螢光蛋白 螢光dye HRP Biotin等) 只要一個步驟 回收100%。這個方法克服二抗所造成的背景值過高及Host來源不同所造成Crosstalk的現象.
Lightning-link產品種類:
抗體標誌產品，可分為五大類產品。(此部份僅介紹酵素型--抗體標誌產品)
1. 酵素型標誌產品；HRP、AP、Glucose Oxidase等。
2. 螢光蛋白標誌產品；APC、PE、PerCP、RPE等螢光蛋白。
3. Biotin/Streptavidin標誌產品；Avidin、Streptavidin、Biotin等。
4. Tandem螢光標誌產品；應用FRET原理，使螢光emission於較遠的波段，例如APC/Cy5.5、PE/Cy7、PE/Cy5.5等。
Fluorescent dye Labeling Kits 螢光Dye----抗體標誌套組
Fluorescent Dye Labeling Kits
Lightning-link螢光蛋白抗體標誌---期刊發表:
Lightning-Link Cy3 ---期刊發表
Lightning-Link Cy3
- Heparin inhibits the interaction of DNA topoisomerase I (topo-I)/anti-topo-I immune complexes with heparan sulfate on dermal fibroblasts - Arthritis & Rheumatism (2011)
- Intracellular trafficking and fate of chimeric adenovirus 5/F35 in human B lymphocytes - The Journal of Gene Medicine (2011)
- Suppression of protein phosphatase 2A activity enhances Ad5/F35 adenovirus transduction efficiency in normal human B lymphocytes and in Raji cells - J Immunol Methods (2011)
Lightning-Link Cy5---期刊發表
Lightning-Link Cy5
- Physical Characterization of the 'Immunosignaturing Effect' - Molecular and cellular proteomics (2012)
- Interleukin-1β Interferes with Epidermal Homeostasis through Induction of Insulin Resistance: Implications for Psoriasis Pathogenesis - Journal of Investigative Dermatology (2012)
Lightning-Link Atto 488 ---期刊發表
Lightning-Link Atto 488
- TCF7L2 rs7903146 impairs islet function and morphology in non-diabetic individuals - Diabetologia (2012)
- The Repetitive Oligopeptide Sequences Modulate Cytopathic Potency but Are Not Crucial for Cellular Uptake of Clostridium difficile Toxin A – PLoS One (2011)
- The N-Terminal Amphipathic Helix of the Topological Specificity Factor MinE Is Associated with Shaping Membrane Curvature - PLoS One (2011)
- Autophagy is involved in the early step of Japanese encephalitis virus infection - Microbes Infect. (2011)
- A novel expression and purification system for the production of enzymatic and biologically active human granzyme B - J Immunol Methods (2011)
- Coxsackievirus B3-Induced Cellular Protrusions: Structural Characteristics and Functional Competence - Journal of Virology (2011)
- The N-Terminal Amphipathic Helix of the Topological Specificity Factor MinE Is Associated with Shaping Membrane Curvature - PLoS One (2011)
- Vimentin binding is critical for infection by the virulent strain of Japanese encephalitis virus - Cellular Microbiology (2011)
Lightning-Link Atto 565 ---期刊發表
Lightning-Link Atto 565
Lightning-Link Atto 633 ---期刊發表
Lightning-Link Atto 633
- The utility of a high-throughput scanning biosensor in the detection of the pancreatic cancer marker ULBP2 - Biosensors and Bioelectronics (2012)
- Pneumocystis Infection in an Immunocompetent Host Can Promote Collateral Sensitization to Respiratory Antigens - Infect Immun. (2011)
- Binding Kinetics of Biomolecule Interaction at Ultralow Concentrations Based on Gold Nanoparticle Enhancement - Anal. Chem. (2011)
- Discrimination of Breast Cancer by Measuring Prostate-Specific Antigen Levels in Women's Serum - Anal. Chem. (2011)
- Structural basis of anthrax edema factor neutralization by a neutralizing antibody - Biochemical and Biophysical Research Communications (2011)
- Flow cytometric detection of myeloperoxidase in horse neutrophils: a novel technique in equine diagnostic research - Veterinary Immunology and Immunopathology (2011)
- Detection of swine-origin influenza A (H1N1) viruses using a localized surface plasmon coupled fluorescence fiber-optic biosensor - Biosensors and Bioelectronics (2010)
Lightning-Link PerCp/Cy5.5 ---期刊發表
Lightning-Link PerCP/Cy5.5
- Thymoquinone-induced Neu4 sialidase activates NFκB in macrophage cells and pro-inflammatory cytokines in vivo - Glycoconjugate Journal (2011)
Lightning-Link PE/Cy5 ---期刊發表
Lightning-Link PE/Cy5
- Preparation of epoxy-based macroporous monolithic columns for the fast and efficient immunofiltration of Staphylococcus aureus - Journal of Separation Science (2011)
- The Repetitive Oligopeptide Sequences Modulate Cytopathic Potency but Are Not Crucial for Cellular Uptake of Clostridium difficile Toxin A - PLoS One (2011)
- Association of Tat with Promoters of PTEN and PP2A Subunits Is Key to Transcriptional Activation of Apoptotic Pathways in HIV-Infected CD4+ T Cells - PLoS Pathogens (2010)
Lightning-Link APC/Cy7 ---期刊發表
Lightning-Link APC/Cy7
Lightning-Link PE/Cy5.5 ---期刊發表
Lightning-Link PE/Cy5.5
- The Repetitive Oligopeptide Sequences Modulate Cytopathic Potency but Are Not Crucial for Cellular Uptake of Clostridium difficile Toxin A – PLoS One (2011)
- Infection of Hematopoietic Progenitor Cells by HIV-1 - University of Michigan Thesis (2011)
- Distinctive immunoregulatory effects of adenosine on T cells of older humans - The FASEB Journal (2010)
- HIV–1 Infects Multipotent Progenitor Cells Causing Cell Death and Establishing Latent Cellular Reservoirs – Nat Med (2010)
Lightning-Link Rhodamine---期刊發表
Lightning-Link Rhodamine
- Cytosolic DNA Triggers Inflammasome Activation in Keratinocytes in Psoriatic Lesions - Sci Transl Med (2011)
Lightning-Link FITC---期刊發表
Lightning-Link Fluorescein
- Photocrosslinkable dextran hydrogel films as substrates for osteoblast and endothelial cell growth - Journal of Materials Chemistry (2012)
- The Glycosylation Pattern of Common Allergens: The Recognition and Uptake of Der p 1 by Epithelial and Dendritic Cells Is Carbohydrate Dependent - PLoS One (2012)
- ARL4A acts with GCC185 to modulate Golgi complex organization - Journal of Cell (2011)
- Fluorescent Labeling of Antibody Fragments Using Split GFP - PLoS One (2011)
- Galectin-1 and Galectin-8 Have Redundant Roles in Promoting Plasma Cell Formation - Journal of Immunology (2011)
- Proliferative potential and phenotypic analysis of long-term cultivated human granulosa cells initiated by addition of follicular fluid - Journal of Assisted Reproduction and Genetics (2011)
- Restricted access of proteins to mannan polysaccharides in intact plant cell walls - The Plant Journal (2010)
【供應廠商】萊恩生物科技有限公司
【公司電話】04-2523-9639
【業務專員】陳俊豪 0937-229803
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